Featured Publications

Grayscale-to-Color: Scalable Fabrication of Custom Multispectral Filter Arrays.

Posted on , Published to PubMed

C Williams, G S D Gordon, T D Wilkinson and S E Bohndiek (2019) ACS Photonics. 18;6(12):3132-3141. doi: 10.1021/acsphotonics.9b01196

Multispectral imaging is an exciting technology that can be applied to extract morphological (spatial) and biochemical (spectral) information from tumours. Typically, each multispectral imaging application requires a specific spectral range and number of spectral bands, which is challenging for conventional manufacturing processes. We present here a versatile, wafer-scale framework for producing highly efficient, narrowband and customisable transmissive multispectral imaging filter arrays. We envisage that the process can be used to fabricate custom multispectral imaging cameras, paving the way in the future for widespread application of the technology in biomedicine, including for endoscopic and intraoperative imaging.


A clinically translatable hyperspectral endoscopy (HySE) system for imaging the gastrointestinal tract

Posted on , Published to Nature Communications

J Yoon, J Joseph, D J Waterhouse, A S Luthman, G S D Gordon, M di Pietro, W Januszewicz, R C Fitzgerald and S E Bohndiek, Nature Communications, 10, Article number: 1902 (2019)

In this work, we demonstrate a hyperspectral endoscope (HySE) that simultaneously records intrinsically co-registered hyperspectral and standard-of-care white light images, which allows image distortions to be compensated computationally and an accurate hyperspectral data cube to be reconstructed as the endoscope moves in the lumen. Evaluation of HySE performance shows excellent spatial, spectral and temporal resolution and high colour fidelity. Application of HySE enables: quantification of blood oxygenation levels in tissue mimicking phantoms; differentiation of spectral profiles from normal and pathological ex vivo human tissues; and recording of hyperspectral data under freehand motion within an intact ex vivo pig oesophagus model. HySE therefore shows potential for enabling HSI in clinical endoscopy.

Oxygen-enhanced and dynamic contrast-enhanced optoacoustic tomography provide surrogate biomarkers of tumour vascular function, hypoxia and necrosis.

Posted on , Published to PubMed

M. R. Tomaszewski, M Gehrung, J Joseph, I Quiros Gonzalez, J A Disselhorst and S E Bohndiek, Cancer Res. 78(20):5980-5991 (2018).

Oxygen Enhanced Optoacoustic Tomography (OE-OT) is a technique we developed and reported in 2017, exploiting gas challenge to provide high contrast insight into tumour vascular function. In this work, we show that OE-OT in combination with Dynamic Contrast Enhanced OT (DCE-OT), which relies on fluorescent contrast injection, can be used to accurately measure tumour oxygenation, maturity of the vascular network and the viability of the tumour tissue.

Graphitic and oxidised high pressure high temperature (HPHT) nanodiamonds induce differential biological responses in breast cancer cell lines.

Posted on , Published to PubMed

B Woodhams, L Ansel-Bollepalli, j Surmacki, H Knowles, L Maggini, M de Volder, M Atatüre and S E Bohndiek. (2018) Epub ahead of print at Nanoscale.

Nanodiamonds have demonstrated potential as powerful sensors in biomedicine. However, their translation into routine use requires a comprehensive understanding of their effect on the biological system being interrogated.  In this paper we assessed the biological impact of graphitic and oxidized nanodiamond surfaces. We show for the first time that oxidized nanodiamonds possess improved biocompatibility compared to graphitic nanodiamonds in breast cancer cell lines, with graphitic nanodiamonds inducing higher levels of oxidative stress despite lower uptake.

Processing methods for photoacoustic Doppler flowmetry with a clinical ultrasound scanner

Posted on , Published to PubMed

Thore M. Bücking; Pim J. van den Berg; Stavroula Balabani; Wiendelt Steenbergen; Paul C. Beard; Joanna Brunker

In this work, researchers announce new advances in measuring blood flow velocity in deep tissue. Blood flow speed is a critical element in assessing tissue functionality as well as diagnosing diseases, and photoacoustic flowmetry (PAF) is already acknowledged as a promising technique for deep tissue measurement of blood flow velocity. The new work demonstrates successful use of a handheld ultrasound probe common in clinical settings, paving the way to explore the feasibility of measuring flow in a physiologically realistic situation.

Oxygen enhanced photoacoustic imaging reveals vascular dynamics in murine models of prostate cancer.

Posted on , Published to PubMed

M R Tomaszewski, I Quiros-Gonzalez, J O'Connor, O Abeyakoon, G Parker, K Williams, F Gilbert and S E Bohndiek (2017) Theranostics. 7 (11) 2900-2915.

Optoacoustic tomography (OT) is an emerging clinical imaging modality that provides static images of endogenous haemoglobin concentration and oxygenation. Here, we demonstrate oxygen enhanced (OE)-OT, exploiting an oxygen gas challenge to visualise the spatiotemporal heterogeneity of tumour vascular function.

Wide field fluorescence hyperspectral imaging (fHSI) for biomedical applications.

Posted on , Published to PubMed

A S N Luthman, S Dumitru, I Quiros-Gonzalez, J Joseph and S E Bohndiek. (2017) J. Biophotonics. 10 (6-7) 840-853.

Spectrally resolved detector arrays are an exciting new technology that allows spectral information to be recorded by conventional CMOS cameras (akin to those found in your smartphone). Here, we show that an SRDA can in fact be used to detect fluorescence signals for biomedical imaging applications.

Design and validation of a near-infrared fluorescence endoscope for detection of early esophageal malignancy

Posted on , Published to PubMed

D J Waterhouse, J Joseph, A A Neves, M di Pietro, K M Brindle, R C Fitzgerald and S E Bohndiek (2016) J. Biomed. Opt. 21(8), 084001.

In this work, we created a bimodal endoscope to reveal the binding of a near infrared fluorescent dye sprayed onto oesophageal tissue for detection of early malignancy.

Summary Figure

Single-pixel phase-corrected fiber bundle endomicroscopy with lensless focussing capability.

Posted on , Published to IEEExplore

G S D Gordon, J Joseph, S E Bohndiek and T D Wilkinson (2015) J Lightwave Technol. 33 16 3419-3425.

The characterisation of modal propagation in fibre bundles is performed here to achieve lensless focusing of imaging data. This is a first step towards performing phase retrieval and lensless focusing during clinical endoscopy using a fibre bundle.

Photoacoustic tomography detects early vessel regression and normalization during ovarian tumor response to the anti-angiogenic therapy Trebananib.

Posted on , Published to PubMed

S E Bohndiek, Laura S Sasportas, Steve Machtaler, Jesse V Jokerst, Sharon Hori and Sanjiv S Gambhir (2015) J Nucl Med. Accepted.

We were able to show here for the first time that optoacoustic imaging is able to detect both the vessel regression and normalisation that is commonly observed with anti-angiogenic therapy. This opens the possibility of using optoacoustic imaging to schedule combination therapy with cytotoxic drugs.

Figure 3 - Photoacoustic Images

A small animal Raman instrument for rapid, wide-area, spectroscopic imaging

Posted on , Published to PubMed

S E Bohndiek, A Wagadarikar, C L Zavaleta, D Van De Sompel, E Garai, J V Jokerst, S Yazdanfar and S S Gambhir (2013) Proc Natl Acad Sci USA 110 (30) 12408-12413

Recently, we developed a dedicated instrument for high throughput Raman spectroscopy, accelerating preclinical studies with this technique and hence improving the long-term prospects for clinical translation.


Hyperpolarized [1-13C]-Ascorbic and Dehydroascorbic Acid: Vitamin C as a probe for imaging redox status in vivo

Posted on , Published to PubMed

S E Bohndiek, M I Kettunen, D Hu, B W C Kennedy, J Boren, F A Gallagher and K M Brindle (2011) J Am Chem Soc A 133 (30) 11795-11801.

In this study, we demonstrated for the first time that vitamin C could be hyperpolarized and also used as a contrast agent to detect redox state noninvasively in living subjects.

2011_AATOC graphic